MED8 antibody (N-Term)

Catalog No. ABIN6741137

Product Details anti-MED8 Antibody

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED8 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human MED8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human MED8 (Q96G25-2, NP_443109). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%), Opossum, Platypus, Xenopus (92%), Zebrafish (85%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED8

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Alternative Name: MED8 (MED8 Products)

Synonyms: ARC32 antibody, 2210021A15Rik antibody, AB041805 antibody, MNCb-2386 antibody, zgc:76877 antibody, med8-a antibody, mediator complex subunit 8 antibody, mediator complex subunit 8 L homeolog antibody, component of RNA polymerase II antibody, mediator complex subunit Med8 antibody, MED8 antibody, Med8 antibody, med8 antibody, med8.L antibody

Background: Name/Gene ID: MED8

Synonyms: MED8, ARC32, Mediator complex subunit 8

Gene ID: 112950

NCBI Accession: NP_443109

UniProt: Q96G25

Pathways: Regulation of Lipid Metabolism by PPARalpha