IDH2 antibody (AA 144-193)

Catalog No. ABIN6741027

Product Details anti-IDH2 Antibody

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Binding Specificity: AA 144-193

Reactivity: Human, Mouse, Rat, Pig, Monkey, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Bat, Hamster, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human IDH2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human IDH2 (P48735, NP_002159). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Stickleback, Zebrafish, Sea squirt, Slime mold (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH / IDH2 (IDH2 Products)

Background: Name/Gene ID: IDH2

Synonyms: IDH2, D2HGA2, ICD-M, IDP, IDPM, MNADP-IDH, NADP(+)-specific ICDH, IDH, IDHM, Isocitrate dehydrogenase, Oxalosuccinate decarboxylase

Gene ID: 3418

NCBI Accession: NP_002159

UniProt: P48735

Pathways: Warburg Effect