PI3 antibody (AA 36-85)

Catalog No. ABIN6740924

Product Details anti-PI3 Antibody

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Binding Specificity: AA 36-85

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PI3

Predicted Reactivity: Percent identity by BLAST analysis: Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human PI3 (P19957, NP_002629). Percent identity by BLAST analysis: Human, Chimpanzee (100%), Gorilla (92%), Marmoset (85%), Bovine (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Alternative Name: PI3 (PI3 Products)

Synonyms: ESI antibody, SKALP antibody, WAP3 antibody, WFDC14 antibody, cementoin antibody, trappin-2 antibody, TRAPPIN-2 antibody, elafin antibody, peptidase inhibitor 3 antibody, peptidase inhibitor 3, skin-derived (SKALP) antibody, PI3 antibody

Background: Name/Gene ID: PI3

Synonyms: PI3, Cementoin, Elafin, Elastase-specific inhibitor, ESI, PI-3, Protease inhibitor WAP3, Trappin-2, Peptidase inhibitor 3, Protease inhibitor 3, SKALP, WAP3, WFDC14, Pre-elafin

Gene ID: 5266

NCBI Accession: NP_002629

UniProt: P19957