UBE3B antibody (AA 720-769)

Catalog No. ABIN6740821

Product Details anti-UBE3B Antibody

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Binding Specificity: AA 720-769

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Pig, Bat, Chicken, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human UBE3B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa720-769 of human UBE3B (Q7Z3V4, NP_569733). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Stickleback, Zebrafish (92%), Drosophila (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE3B

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Alternative Name: UBE3B (UBE3B Products)

Synonyms: si:dkey-189p24.3 antibody, BPIDS antibody, AI449831 antibody, AU020130 antibody, ubiquitin protein ligase E3B antibody, ubiquitin-protein ligase E3B antibody, ubiquitin protein ligase E3B L homeolog antibody, UBE3B antibody, ube3b antibody, LOC581811 antibody, ube3b.L antibody, Ube3b antibody

Background: Name/Gene ID: UBE3B

Synonyms: UBE3B, Ubiquitin-protein ligase E3B, Ubiquitin protein ligase E3B

Gene ID: 89910

NCBI Accession: NP_569733

UniProt: Q7Z3V4