MARCH4 antibody (AA 36-85)

Catalog No. ABIN6740812

Product Details anti-MARCH4 Antibody

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human MARCH4

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human MARCH4 (Q9P2E8, NP_065865). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Horse, Opossum (100%), Panda, Dog, Bat, Guinea pig (92%), Mouse, Rat, Hamster, Rabbit (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: RNF174 / MARCH4 (MARCH4 Products)

Synonyms: MARCH-IV antibody, RNF174 antibody, BC056494 antibody, mKIAA1399 antibody, MARCH4 antibody, si:ch211-239d11.2 antibody, membrane associated ring-CH-type finger 4 antibody, membrane-associated ring finger (C3HC4) 4 antibody, MARCH4 antibody, March4 antibody, march4 antibody

Background: Name/Gene ID: MARCH4

Synonyms: MARCH4, RING finger protein 174, KIAA1399, MARCH-IV, RNF174

Gene ID: 57574

NCBI Accession: NP_065865

UniProt: Q9P2E8