ANO1 antibody (AA 828-877)

Catalog No. ABIN6740769

Product Details anti-ANO1 Antibody

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Binding Specificity: AA 828-877

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ANO1 / TMEM16A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa828-877 of human ANO1 (Q5XXA6, NP_060513). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Horse, Guinea pig (100%), Marmoset, Zebra finch (92%), Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: ANO1 / DOG1 / TMEM16A (ANO1 Products)

Synonyms: DOG1 antibody, ORAOV2 antibody, TAOS2 antibody, TMEM16A antibody, Tmem16a antibody, anoctamin 1 antibody, anoctamin 1, calcium activated chloride channel antibody, Ano1 antibody, ANO1 antibody

Background: Name/Gene ID: ANO1

Synonyms: ANO1, Oral cancer overexpressed 2, Transmembrane protein 16A, ORAOV2, Anoctamin-1, DOG1, TAOS2, TMEM16A

Gene ID: 55107

NCBI Accession: NP_060513

UniProt: Q5XXA6