CHRNA4 antibody (AA 36-85)

Catalog No. ABIN6740407

Product Details anti-CHRNA4 Antibody

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Chicken, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRNA4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human CHRNA4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Guinea pig (100%) Rabbit, Zebrafish (92%) Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human CHRNA4 (P43681, NP_000735). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Ferret, Rabbit, Lizard, Zebrafish (92%), Bat (91%), Gibbon, Dog, Bovine, Horse, Pig, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHRNA4

Target: CHRNA4 (Cholinergic Receptor, Nicotinic, alpha 4 (CHRNA4))

Alternative Name: CHRNA4 / NACHR (CHRNA4 Products)

Synonyms: NACHRA4 antibody, BFNC antibody, EBN antibody, EBN1 antibody, NACHR antibody, NACRA4 antibody, NARAC antibody, Acra-4 antibody, Acra4 antibody, ENFL1 antibody, neuronal acetylcholine receptor subunit alpha-4 antibody, neuronal acetylcholine receptor subunit alpha-4-like antibody, cholinergic receptor nicotinic alpha 4 subunit antibody, cholinergic receptor, nicotinic, alpha 4b antibody, cholinergic receptor, nicotinic, alpha polypeptide 4 antibody, CpipJ_CPIJ002434 antibody, LOC100368057 antibody, CHRNA4 antibody, chrna4b antibody, Chrna4 antibody

Background: Name/Gene ID: CHRNA4
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA4, BFNC, AChR-alpha4, Acra-4, EBN, EBN1, NACHR, NAChR alpha 4, NACRA4, NACHRA4, ENFL1

Gene ID: 1137

NCBI Accession: NP_000735

UniProt: P43681

Pathways: Synaptic Membrane