PEDF antibody (N-Term)
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- Target See all PEDF (SERPINF1) Antibodies
- PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Pig, Dog, Guinea Pig, Horse, Sheep, Bat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PEDF antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SERPINF1 / PEDF
- Predicted Reactivity
- Percent identity by BLAST analysis: Human (100%) Mouse, Sheep (86%) Horse, Pig, Guinea pig (80%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human SERPINF1 (P36955, NP_002606). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Bat (100%), Gibbon (93%), Galago (92%), Marmoset, Mouse, Sheep, Bovine (86%), Opossum, Platypus (81%), Elephant, Horse, Pig, Guinea pig (80%), Rat (75%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product SERPINF1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (4 - 8 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4-8 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 50 μL sterile ddH2O.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS buffer with 2 % sucrose
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
- Alternative Name
- SERPINF1 / PEDF (SERPINF1 Products)
- Synonyms
- EPC-1 antibody, OI12 antibody, OI6 antibody, PEDF antibody, MGC82984 antibody, SERPINF1 antibody, AI195227 antibody, Pedf antibody, Pedfl antibody, Sdf3 antibody, Dmrs91 antibody, zgc:92072 antibody, serpin family F member 1 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog antibody, serine (or cysteine) peptidase inhibitor, clade F, member 1 antibody, SERPINF1 antibody, serpinf1 antibody, serpinf1.L antibody, Serpinf1 antibody
- Background
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Name/Gene ID: SERPINF1
Synonyms: SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6 - Gene ID
- 5176
- NCBI Accession
- NP_002606
- UniProt
- P36955
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