SPO11 antibody (AA 110-159)

Catalog No. ABIN6739020

Product Details anti-SPO11 Antibody

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Binding Specificity: AA 110-159

Reactivity: Human, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio), Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPO11 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human SPO11

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Pig, Guinea pig (100%) Mouse, Rat, Rabbit, Horse (92%) Chicken (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of human SPO11 (Q9Y5K1, NP_036576). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Bat, Pig, Opossum, Guinea pig (100%), Mouse, Rat, Rabbit, Horse, Zebra finch, Platypus (92%), Turkey, Chicken (84%), Stickleback (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SPO11

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Alternative Name: SPO11 (SPO11 Products)

Synonyms: CT35 antibody, SPATA43 antibody, TOPVIA antibody, zgc:77876 antibody, AI449549 antibody, Meiotic recombination protein spo-11 antibody, SPO11, initiator of meiotic double stranded breaks antibody, SPO11 meiotic protein covalently bound to DSB antibody, spo-11 antibody, SPO11 antibody, Spo11 antibody, spo11 antibody

Background: Name/Gene ID: SPO11

Synonyms: SPO11, Cancer/testis antigen 35, CT35

Gene ID: 23626

NCBI Accession: NP_036576

UniProt: Q9Y5K1