NAD-ME antibody (AA 38-87)

Catalog No. ABIN6738732

Product Details anti-NAD-ME Antibody

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Binding Specificity: AA 38-87

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAD-ME antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ME2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit (100%) Horse, Pig (92%) Xenopus (91%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of human ME2 (P23368, NP_002387). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Bovine, Rabbit, Platypus (100%), Elephant, Panda, Bat, Horse, Pig, Opossum, Guinea pig (92%), Zebra finch, Xenopus (91%), Dog (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NAD-ME

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Alternative Name: ME2 / Malate Dehydrogenase 2 (NAD-ME Products)

Synonyms: ODS1 antibody, AW120568 antibody, D030040L20Rik antibody, NAD-ME antibody, zgc:100941 antibody, malic enzyme 2 antibody, malic enzyme 2, NAD(+)-dependent, mitochondrial antibody, malic enzyme 2, NAD(+)-dependent, mitochondrial S homeolog antibody, ME2 antibody, Me2 antibody, me2.S antibody, me2 antibody

Background: Name/Gene ID: ME2

Synonyms: ME2, Malate dehydrogenase, Pyruvic-malic carboxylase, Malic enzyme 2, NAD-ME, ODS1

Gene ID: 4200

NCBI Accession: NP_002387

UniProt: P23368

Pathways: Production of Molecular Mediator of Immune Response