HLA-F antibody (AA 110-159)

Catalog No. ABIN6738557

Product Details anti-HLA-F Antibody

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Binding Specificity: AA 110-159

Reactivity: Human, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-F antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human HLA-F

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of human HLA-F (P30511, NP_001091949). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Bovine (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HLA-F

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Alternative Name: HLA-F (HLAF Products)

Synonyms: HLAF antibody, CDA12 antibody, HLA-5.4 antibody, HLA-CDA12 antibody, major histocompatibility complex, class I, F antibody, HLA-F antibody

Background: Name/Gene ID: HLA-F

Synonyms: HLA-F, CDA12, HLAF, HLA-CDA12, MHC class I antigen F, HLA F antigen, MHC class Ib antigen, HLA-5.4, Leukocyte antigen F

Gene ID: 3134

NCBI Accession: NP_001091949

UniProt: P30511

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response