PRAS40 antibody (AA 202-251)

Catalog No. ABIN6738417

Product Details anti-PRAS40 Antibody

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Binding Specificity: AA 202-251

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human AKT1S1 / PRAS40

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Bovine, Pig, Guinea pig (100%) Dog (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa202-251 of human AKT1S1 (Q96B36, NP_115751). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Bat, Horse, Pig, Guinea pig (100%), Dog (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1 / PRAS40 (AKT1S1 Products)

Synonyms: MGC81452 antibody, lobe antibody, pras40 antibody, fb34a04 antibody, wu:fb34a04 antibody, Lobe antibody, PRAS40 antibody, 1110012J22Rik antibody, AI227026 antibody, Lobel antibody, AKT1 substrate 1 (proline rich) S homeolog antibody, AKT1 substrate 1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 (proline-rich) antibody, akt1s1.S antibody, AKT1S1 antibody, akt1s1 antibody, Akt1s1 antibody

Background: Name/Gene ID: AKT1S1

Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe

Gene ID: 84335

NCBI Accession: NP_115751

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect