Reticulon 4 antibody (AA 81-130)

Catalog No. ABIN6738273

Product Details anti-Reticulon 4 Antibody

Target: Reticulon 4 (RTN4)

Binding Specificity: AA 81-130

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Monkey, Horse, Guinea Pig, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human RTN4 / Nogo

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa81-130 of human RTN4 (Q7L7Q5, NP_008939). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Elephant, Opossum, Platypus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4 / Nogo (RTN4 Products)

Background: Name/Gene ID: RTN4
Family: Reticulon

Synonyms: RTN4, Human NogoA, My043 protein, Neurite outgrowth inhibitor, NOGOC, NSP-CL, Nogo protein, KIAA0886, Reticulon 5, Reticulon-4, Reticulon-5, Reticulon 4, RTN4-B1, RTN-X, RTN4-A, RTN4-B2, RTN4-C, Nbla00271, Neurite growth inhibitor 220, NI220/250, NOGO-A, Nogo-C, ASY, Foocen, Nbla10545, NOGO, Nogo-B, NSP

Gene ID: 57142

NCBI Accession: NP_008939

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome