Reticulon 4 antibody (AA 254-303)

Catalog No. ABIN6738272

Product Details anti-Reticulon 4 Antibody

Target: Reticulon 4 (RTN4)

Binding Specificity: AA 254-303

Reactivity: Human, Mouse, Rat, Rabbit, Sheep, Cow, Dog, Monkey, Horse, Pig, Bat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human RTN4 / Nogo

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human RTN4 (Q9NQC3, NP_997403). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Turkey, Chicken (100%), Opossum, Guinea pig, Sparrow, Lizard (92%), Platypus, Xenopus, Sablefish, Stickleback, Medaka (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4 / Nogo (RTN4 Products)

Synonyms: ASY antibody, NI220/250 antibody, NOGO antibody, NOGO-A antibody, NOGOC antibody, NSP antibody, NSP-CL antibody, Nbla00271 antibody, Nbla10545 antibody, Nogo-B antibody, Nogo-C antibody, RTN-X antibody, RTN4-A antibody, RTN4-B1 antibody, RTN4-B2 antibody, RTN4-C antibody, RTN4-Cw antibody, DKFZp459C0314 antibody, 1110020G17Rik antibody, AA407876 antibody, AA409940 antibody, AA960376 antibody, C130026I10Rik antibody, NgA antibody, Nogo-A antibody, mKIAA0886 antibody, mKIAA4153 antibody, NI-250 antibody, Nogo antibody, Vp20 antibody, r antibody, rat N antibody, rat NogoA antibody, reticulon-4 antibody, nogo antibody, rtn4 antibody, wu:fb17a02 antibody, wu:fb22f12 antibody, wu:fd61a07 antibody, zgc:92163 antibody, reticulon 4 antibody, reticulon 4a antibody, RTN4 antibody, rtn4 antibody, Rtn4 antibody, rtn4a antibody

Background: Name/Gene ID: RTN4
Family: Reticulon

Synonyms: RTN4, Human NogoA, My043 protein, Neurite outgrowth inhibitor, NOGOC, NSP-CL, Nogo protein, KIAA0886, Reticulon 5, Reticulon-4, Reticulon-5, Reticulon 4, RTN4-B1, RTN-X, RTN4-A, RTN4-B2, RTN4-C, Nbla00271, Neurite growth inhibitor 220, NI220/250, NOGO-A, Nogo-C, ASY, Foocen, Nbla10545, NOGO, Nogo-B, NSP

Gene ID: 57142

NCBI Accession: NP_997403

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome