RAD18 antibody (AA 197-246)

Catalog No. ABIN6738067

Product Details anti-RAD18 Antibody

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Binding Specificity: AA 197-246

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human RAD18

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%) Dog, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa197-246 of human RAD18 (Q9NS91, NP_064550). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig (100%), Marmoset, Hamster, Panda, Opossum, Guinea pig (92%), Galago, Dog, Turkey, Zebra finch, Chicken, Sablefish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: RAD18 (RAD18 Products)

Synonyms: RNF73 antibody, 2810024C04Rik antibody, Rad18sc antibody, zgc:55577 antibody, RAD18 antibody, DDBDRAFT_0218120 antibody, DDBDRAFT_0304878 antibody, DDB_0218120 antibody, DDB_0304878 antibody, DKFZp469H0810 antibody, RAD18, E3 ubiquitin protein ligase antibody, RAD18 E3 ubiquitin protein ligase antibody, E3 ubiquitin-protein ligase RAD18 antibody, E3 ubiquitin-protein ligase antibody, DNA repair protein and ATPase antibody, C3HC4-type zinc finger-containing protein antibody, RAD18 antibody, Rad18 antibody, rad18 antibody

Background: Name/Gene ID: RAD18

Synonyms: RAD18, HHR18, HRAD18, RNF73, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73

Gene ID: 56852

NCBI Accession: NP_064550