POLR2I antibody (N-Term)

Catalog No. ABIN6737851

Product Details anti-POLR2I Antibody

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig, Monkey, Zebrafish (Danio rerio), Bat, Drosophila melanogaster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2I antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human POLR2I

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human POLR2I (P36954, NP_006224). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Xenopus, Trout, Catfish, Stickleback, Zebrafish, Drosophila, Beetle (100%), Turkey (92%), Arabidopsis (91%), Slime mold, Rice, Grape (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR2I

Target: POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))

Alternative Name: POLR2I (POLR2I Products)

Synonyms: RPB9 antibody, hRPB14.5 antibody, GB18029 antibody, RPB14.5 antibody, 2810002B19Rik antibody, zgc:103515 antibody, RNA polymerase II subunit I antibody, DNA-directed RNA polymerase II subunit RPB9 antibody, DNA-directed RNA polymerase II 14.5 kDa polypeptide antibody, polymerase (RNA) II (DNA directed) polypeptide I antibody, polymerase (RNA) II subunit I L homeolog antibody, POLR2I antibody, LOC412547 antibody, LOC100282092 antibody, Polr2i antibody, polr2i antibody, polr2i.L antibody

Background: Name/Gene ID: POLR2I

Synonyms: POLR2I, HRPB14.5, RNA polymerase II subunit B9, RPB14.5, RPB9

Gene ID: 5438

NCBI Accession: NP_006224

UniProt: P36954

Pathways: Regulatory RNA Pathways