MED16 antibody (AA 171-220)

Catalog No. ABIN6737649

Product Details anti-MED16 Antibody

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Binding Specificity: AA 171-220

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED16 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human MED16 / THRAP5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa171-220 of human MED16 (Q9Y2X0, NP_005472). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Chicken (92%), Zebra finch, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED16

Target: MED16 (Mediator Complex Subunit 16 (MED16))

Alternative Name: MED16 / THRAP5 (MED16 Products)

Synonyms: THRAP5 antibody, MED16 antibody, DRIP92 antibody, TRAP95 antibody, 95kDa antibody, A630083L04 antibody, Thrap5 antibody, Trap95 antibody, mediator complex subunit 16 antibody, mediator complex subunit 16 L homeolog antibody, MED16 antibody, Med16 antibody, med16.L antibody

Background: Name/Gene ID: MED16

Synonyms: MED16, DRIP92, Mediator complex subunit 16, TRAP95, THRAP5

Gene ID: 10025

NCBI Accession: NP_005472

UniProt: Q9Y2X0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha