HDLBP antibody (N-Term)

Catalog No. ABIN6736763

Product Details anti-HDLBP Antibody

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Binding Specificity: N-Term

Reactivity: Human, Rat, Horse, Rabbit, Dog, Guinea Pig, Bat, Hamster, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDLBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HDLBP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Pig, Guinea pig (100%) Mouse, Bovine, Chicken (92%) Xenopus (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HDLBP (Q00341, NP_005327). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Rat, Hamster, Elephant, Dog, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Mouse, Bovine, Opossum, Turkey, Chicken (92%), Platypus, Lizard (84%), Xenopus (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HDLBP

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Alternative Name: HDLBP / Vigilin (HDLBP Products)

Synonyms: HBP antibody, PRO2900 antibody, VGL antibody, 1110005P14Rik antibody, AA960365 antibody, AI118566 antibody, D1Ertd101e antibody, HDLBP antibody, vigilin antibody, cb591 antibody, cb811 antibody, hdlbp antibody, wu:fb36d01 antibody, wu:fb94h08 antibody, wu:fc23d06 antibody, wu:fc75h07 antibody, high density lipoprotein binding protein antibody, high density lipoprotein (HDL) binding protein antibody, vigilin antibody, high density lipoprotein binding protein (vigilin) S homeolog antibody, high density lipoprotein binding protein a antibody, HDLBP antibody, Hdlbp antibody, hdlbp.S antibody, hdlbpa antibody

Background: Name/Gene ID: HDLBP

Synonyms: HDLBP, HBP, VGL, HDL-binding protein, PRO2900, Vigilin

Gene ID: 3069

NCBI Accession: NP_005327

UniProt: Q00341