SOX12 antibody (C-Term)

Catalog No. ABIN6736757

Product Details anti-SOX12 Antibody

Target: SOX12 (SRY (Sex Determining Region Y)-Box 12 (SOX12))

Binding Specificity: C-Term

Reactivity: Human, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SOX12

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog (92%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SOX12 (O15370, NP_008874). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%), Galago, Mouse, Rat, Dog, Horse (92%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SOX12

Target: SOX12 (SRY (Sex Determining Region Y)-Box 12 (SOX12))

Alternative Name: SOX12 (SOX12 Products)

Synonyms: SOX22 antibody, sox-k1 antibody, sox-k1-a antibody, sox33 antibody, xSox-33 antibody, xSox-K1 antibody, xSox33 antibody, 2010205A02Rik antibody, SRY-box 12 antibody, SRY box 12 antibody, SRY-box 12 S homeolog antibody, SRY (sex determining region Y)-box 12 antibody, SOX12 antibody, Sox12 antibody, sox12.S antibody

Background: Name/Gene ID: SOX12
Family: Transcription factor

Synonyms: SOX12, Protein SOX-22, SOX-22 protein, SOX22, SRY-related HMG-box gene 22, Sox-12, Transcription factor SOX-12

Gene ID: 6666

NCBI Accession: NP_008874

UniProt: O15370