CoREST antibody (C-Term)

Catalog No. ABIN6736749

Product Details anti-CoREST Antibody

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Dog, Guinea Pig, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CoREST antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RCOR1 / COREST

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog (100%) Horse (92%) Bovine, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human RCOR1 (Q9UKL0, NP_055971). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Dog (100%), Galago, Horse (92%), Bovine, Turkey, Zebra finch, Chicken, Platypus (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CoREST

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Alternative Name: RCOR1 / COREST (RCOR1 Products)

Background: Name/Gene ID: RCOR1

Synonyms: RCOR1, COREST, Protein CoREST, REST corepressor 1, RCOR, KIAA0071, REST corepressor

Gene ID: 23186

NCBI Accession: NP_055971

UniProt: Q9UKL0

Pathways: Regulation of Hormone Metabolic Process, Chromatin Binding