PARP2 antibody (AA 401-450)

Catalog No. ABIN6736743

Product Details anti-PARP2 Antibody

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Binding Specificity: AA 401-450

Reactivity: Human, Mouse, Dog, Cow, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PARP2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat, Guinea pig (91%) Mouse, Dog, Bovine, Rabbit, Horse, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa401-450 of human PARP2 (Q9UGN5, NP_005475). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Marmoset (92%), Rat, Guinea pig (91%), Mouse, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PARP2

Target: PARP2 (Poly (ADP-Ribose) Polymerase 2 (PARP2))

Alternative Name: PARP2 (PARP2 Products)

Synonyms: ADPRT2 antibody, ADPRTL2 antibody, ADPRTL3 antibody, ARTD2 antibody, PARP-2 antibody, pADPRT-2 antibody, Adprt2 antibody, Adprtl2 antibody, Aspartl2 antibody, C78626 antibody, cb996 antibody, adprtl2 antibody, ADPRT-2 antibody, APP antibody, ATPARP1 antibody, PARP1 antibody, POLY(ADP-RIBOSE) POLYMERASE antibody, POLY(ADP-RIBOSE) POLYMERASE 1 antibody, PP antibody, T14P8.19 antibody, T14P8_19 antibody, poly(ADP-ribose) polymerase antibody, poly(ADP-ribose) polymerase 2 antibody, poly(ADP-ribose) polymerase 2 antibody, poly (ADP-ribose) polymerase family, member 2 antibody, poly (ADP-ribose) polymerase 2 antibody, poly(ADP-ribose) polymerase 2 S homeolog antibody, poly(ADP-ribose) polymerase antibody, PARP2 antibody, Parp2 antibody, parp2 antibody, parp2.S antibody

Background: Name/Gene ID: PARP2

Synonyms: PARP2, ADPRT2, ADPRTL2, ADPRTL3, ADPRT-2, ARTD2, HPARP-2, PADPRT-2, PARP-2, Poly(ADP-ribose) synthetase, Poly (ADP-ribose) polymerase 2, Poly[ADP-ribose] synthetase 2, Poly[ADP-ribose] synthase 2, Poly [ADP-ribose] polymerase 2

Gene ID: 10038

NCBI Accession: NP_005475

UniProt: Q9UGN5

Pathways: DNA Damage Repair