NCOR1 antibody (AA 484-533)

Catalog No. ABIN6736737

Product Details anti-NCOR1 Antibody

Target: NCOR1 (Nuclear Receptor Co-Repressor 1 (NCOR1))

Binding Specificity: AA 484-533

Reactivity: Human, Mouse, Cow, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NCOR1 / N-CoR

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine (100%) Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa484-533 of human NCOR1 (Q60974). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Mouse, Elephant, Panda, Bovine, Bat (100%), Monkey, Galago, Horse, Guinea pig (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NCOR1

Target: NCOR1 (Nuclear Receptor Co-Repressor 1 (NCOR1))

Alternative Name: N-CoR / NCOR1 (NCOR1 Products)

Synonyms: ncor antibody, zgc:56355 antibody, 5730405M06Rik antibody, A230020K14Rik antibody, N-CoR antibody, RIP13 antibody, Rxrip13 antibody, mKIAA1047 antibody, N-CoR1 antibody, TRAC1 antibody, hN-CoR antibody, nuclear receptor corepressor 1 antibody, nuclear receptor co-repressor 1 antibody, nuclear receptor corepressor 1 pseudogene antibody, nuclear receptor corepressor 1 S homeolog antibody, ncor1 antibody, NCOR1 antibody, CpipJ_CPIJ002507 antibody, LOC100343278 antibody, Ncor1 antibody, ncor1.S antibody

Background: Name/Gene ID: NCOR1

Synonyms: NCOR1, HCIT529I10, KIAA1047, N-CoR1, TRAC1, HN-CoR, N-CoR, Nuclear co-repressor n-cor, Nuclear receptor corepressor 1

Gene ID: 9611

UniProt: O75376

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process