EOMES antibody (N-Term)

Catalog No. ABIN6736724

Product Details anti-EOMES Antibody

Target: EOMES (Eomesodermin (EOMES))

Binding Specificity: N-Term

Reactivity: Human, Cow, Dog, Guinea Pig, Horse, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EOMES antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EOMES

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (92%) Dog, Bovine, Horse, Pig, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human EOMES (O95936, NP_005433). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%), Mouse (92%), Dog, Bovine, Bat, Elephant, Horse, Pig, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EOMES

Target: EOMES (Eomesodermin (EOMES))

Alternative Name: Eomesodermin / EOMES (EOMES Products)

Synonyms: TBR2 antibody, eom antibody, eomes antibody, EOMES antibody, xeomes antibody, tbr2 antibody, C77258 antibody, TBR-2 antibody, Tbr2 antibody, eomesodermin antibody, eomesodermin homolog a antibody, eomesodermin L homeolog antibody, EOMES antibody, eomesa antibody, Eomes antibody, eomes.L antibody, eomes antibody

Background: Name/Gene ID: EOMES

Synonyms: EOMES, Eomesodermin, Eomesodermin homolog, T box, brain, 2, T-box brain protein 2, T-box brain2, T-brain-2, TBR-2, TBR2

Gene ID: 8320

NCBI Accession: NP_005433

UniProt: O95936

Pathways: Stem Cell Maintenance