Forkhead Box K2 antibody (AA 491-540)

Catalog No. ABIN6736720

Product Details anti-Forkhead Box K2 Antibody

Target: Forkhead Box K2 (FOXK2)

Binding Specificity: AA 491-540

Reactivity: Human, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Forkhead Box K2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FOXK2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (92%) Mouse (90%) Rat, Chicken, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa491-540 of human FOXK2 (Q01167, NP_004505). Percent identity by BLAST analysis: Human, Chimpanzee, Opossum (100%), Gibbon, Monkey, Galago, Marmoset, Bovine, Bat, Horse, Pig (92%), Mouse, Rat, Elephant (90%), Turkey, Chicken, Xenopus (85%), Gorilla (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Forkhead Box K2

Target: Forkhead Box K2 (FOXK2)

Alternative Name: FOXK2 / ILF (FOXK2 Products)

Synonyms: ILF antibody, ILF-1 antibody, ILF1 antibody, wu:fc20a11 antibody, wu:fj68h08 antibody, FOXK2 antibody, 1110054H05Rik antibody, 5730434B08Rik antibody, 6230415M23Rik antibody, Ilf1 antibody, foxk1 antibody, forkhead box K2 antibody, forkhead box protein K2 antibody, forkhead box K2 L homeolog antibody, FOXK2 antibody, foxk2 antibody, LOC719599 antibody, Foxk2 antibody, foxk2.L antibody

Background: Name/Gene ID: FOXK2

Synonyms: FOXK2, ILF1, ILF-1, Forkhead box K2, ILF, Forkhead box protein K2

Gene ID: 3607

NCBI Accession: NP_004505

UniProt: Q01167