EEA1 antibody (AA 307-356)

Catalog No. ABIN6736714

Product Details anti-EEA1 Antibody

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Binding Specificity: AA 307-356

Reactivity: Human, Dog, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EEA1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Guinea pig (92%) Rat, Bovine, Horse (91%) Rabbit (84%) Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa307-356 of human EEA1 (Q15075, NP_003557). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Mouse, Guinea pig, Platypus (92%), Rat, Bovine, Horse (91%), Elephant, Rabbit, Xenopus (84%), Bat, Pig (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1 (EEA1 Products)

Synonyms: MST105 antibody, MSTP105 antibody, ZFYVE2 antibody, im:7151048 antibody, si:ch211-276p10.2 antibody, A430109M19Rik antibody, B230358H09Rik antibody, early endosome antigen 1 antibody, early endosome antigen 1 S homeolog antibody, EEA1 antibody, eea1 antibody, Eea1 antibody, eea1.S antibody

Background: Name/Gene ID: EEA1

Synonyms: EEA1, MST105, MSTP105, ZFYVE2, Early endosome antigen 1

Gene ID: 8411

NCBI Accession: NP_003557

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades