HDAC8 antibody (C-Term)

Catalog No. ABIN6736397

Product Details anti-HDAC8 Antibody

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HDAC8

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse (100%) Guinea pig, Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human HDAC8 (Q9BY41, NP_060956). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse (100%), Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HDAC8

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Alternative Name: HDAC8 (HDAC8 Products)

Background: Name/Gene ID: HDAC8
Family: Histone Deacetylase

Synonyms: HDAC8, CDLS5, HD8, HDACL1, Histone deacetylase 8, MRXS6, RPD3, CDA07, Histone deacetylase-like 1, WTS

Gene ID: 55869

NCBI Accession: NP_060956

UniProt: Q9BY41

Pathways: Cellular Glucan Metabolic Process