GSTM1 antibody (AA 31-80)

Catalog No. ABIN6736393

Product Details anti-GSTM1 Antibody

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Binding Specificity: AA 31-80

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Monkey, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GSTM1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Guinea pig (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa31-80 of human GSTM1 (P46439, NP_000552). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Lizard, Blood fluke (100%), Chimpanzee, Elephant, Pig, Chicken, Xenopus (92%), Sablefish, Zebrafish, Water flea (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GSTM1

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Alternative Name: GSTM1 / mu (GSTM1 Products)

Background: Name/Gene ID: GSTM1

Synonyms: GSTM1, Glutathione S-aryltransferase, GSTM1-1, GSTM1a-1a, GSTM1b-1b, Glutathione S-alkyltransferase, GTH4, GST class-mu 1, GST1, GTM1, H-B, HB subunit 4, MU-1, Glutathione S-transferase M1, Glutathione S-transferase mu 1, GST HB subunit 4

Gene ID: 2944

NCBI Accession: NP_000552

UniProt: P09488

Pathways: Negative Regulation of Transporter Activity