Cx40/GJA5 antibody (AA 86-135)

Catalog No. ABIN6736390

Product Details anti-Cx40/GJA5 Antibody

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Binding Specificity: AA 86-135

Reactivity: Human, Dog, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cx40/GJA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GJA5 / Connexin 40

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa86-135 of human GJA5 (P36382, NP_005257). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Pig (92%), Monkey, Elephant, Panda, Dog, Bovine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Cx40/GJA5

Target: Cx40/GJA5 (GJA5) (Gap Junction Protein, alpha 5, 40kDa (GJA5))

Alternative Name: GJA5 / CX40 / Connexin 40 (GJA5 Products)

Background: Name/Gene ID: GJA5
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA5, Connexin 40, Connexin-40, Gap junction alpha-5 protein, ATFB11, CX40, Gap junction protein alpha 5

Gene ID: 2702

NCBI Accession: NP_005257

UniProt: P36382

Pathways: Cell-Cell Junction Organization