FOXJ1 antibody (N-Term)

Catalog No. ABIN6736382

Product Details anti-FOXJ1 Antibody

Target: FOXJ1 (Forkhead Box J1 (FOXJ1))

Binding Specificity: N-Term

Reactivity: Human, Dog, Cow, Sheep, Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXJ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FOXJ1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine (100%) Mouse, Rat, Rabbit, Horse, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human FOXJ1 (Q92949, NP_001445). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Sheep, Hamster, Dog, Bovine, Bat (100%), Mouse, Rat, Elephant, Panda, Rabbit, Horse, Guinea pig (92%), Marmoset (85%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXJ1

Target: FOXJ1 (Forkhead Box J1 (FOXJ1))

Alternative Name: HFH-4 / FOXJ1 (FOXJ1 Products)

Background: Name/Gene ID: FOXJ1
Family: Transcription factor

Synonyms: FOXJ1, Fork head homologue 4, Forkhead box J1, Forkhead box protein J1, HFH-4, Forkhead-like 13, HFH4, FKHL13

Gene ID: 2302

NCBI Accession: NP_001445

UniProt: Q92949

Pathways: Regulation of Leukocyte Mediated Immunity