FOXI1 antibody (AA 221-270)

Catalog No. ABIN6736381

Product Details anti-FOXI1 Antibody

Target: FOXI1 (Forkhead Box I1 (FOXI1))

Binding Specificity: AA 221-270

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FOXI1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa221-270 of human FOXI1 (Q12951, NP_036320). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Marmoset, Tamarin (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXI1

Target: FOXI1 (Forkhead Box I1 (FOXI1))

Alternative Name: FOXI1 (FOXI1 Products)

Background: Name/Gene ID: FOXI1
Family: Transcription factor

Synonyms: FOXI1, Forkhead-related activator 6, FREAC6, Fork-head homolog-3, Forkhead-like 10, FREAC-6, HNF-3/fork-head homolog 3, HFH-3, FKHL10, Forkhead box protein I1, HFH3, FKH10, Forkhead box I1, HNF-3/fork-head homolog-3

Gene ID: 2299

NCBI Accession: NP_036320

UniProt: Q12951