CHRNA3 antibody (AA 26-75)
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- Target See all CHRNA3 Antibodies
- CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))
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Binding Specificity
- AA 26-75
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CHRNA3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human CHRNA3
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Dog, Rabbit (100%) Bovine, Guinea pig (92%) Mouse, Rat (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa26-75 of human CHRNA3 (P32297, NP_000734). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Ferret, Elephant, Dog, Bovine, Rabbit, Horse (100%), Pig, Opossum, Guinea pig (92%), Platypus (90%), Mouse, Rat, Hamster (85%), Turkey (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CHRNA3 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- After adding water, will consist of PBS buffer with 2 % sucrose
- Concentration
- Lot specific
- Buffer
- Lyophilized.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))
- Alternative Name
- CHRNA3 (CHRNA3 Products)
- Synonyms
- LNCR2 antibody, NACHRA3 antibody, PAOD2 antibody, (a)3 antibody, A730007P14Rik antibody, Acra-3 antibody, Acra3 antibody, cholinergic receptor nicotinic alpha 3 subunit antibody, cholinergic receptor, nicotinic, alpha polypeptide 3 antibody, CHRNA3 antibody, Chrna3 antibody
- Background
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Name/Gene ID: CHRNA3
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel
Synonyms: CHRNA3, AChR-alpha3, Acra-3, LNCR2, NAChR alpha 3, PAOD2, NACHRA3 - Gene ID
- 1136
- NCBI Accession
- NP_000734
- UniProt
- P32297
- Pathways
- Synaptic Membrane
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