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BRD4 antibody (AA 651-700)

BRD4 Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN214729
  • Target See all BRD4 Antibodies
    BRD4 (Bromodomain Containing 4 (BRD4))
    Binding Specificity
    • 16
    • 10
    • 8
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 651-700
    Reactivity
    Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig, Bat
    Host
    • 69
    • 1
    • 1
    Rabbit
    Clonality
    • 62
    • 8
    • 1
    Polyclonal
    Conjugate
    • 39
    • 6
    • 6
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BRD4 antibody is un-conjugated
    Application
    • 44
    • 36
    • 23
    • 14
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human BRD4
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product BRD4 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    After adding water, will consist of PBS buffer with 2 % sucrose
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    BRD4 (Bromodomain Containing 4 (BRD4))
    Alternative Name
    BRD4 (BRD4 Products)
    Synonyms
    brd4-b antibody, BRD4 antibody, fb18g03 antibody, wu:fb18g03 antibody, CAP antibody, HUNK1 antibody, HUNKI antibody, MCAP antibody, Brd5 antibody, WI-11513 antibody, bromodomain containing 4 L homeolog antibody, bromodomain containing 4 antibody, bromodomain-containing protein 4 antibody, brd4.L antibody, BRD4 antibody, brd4 antibody, LOC100409351 antibody, Brd4 antibody
    Background
    Name/Gene ID: BRD4
    Subfamily: BRD
    Family: Protein Kinase

    Synonyms: BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI
    Gene ID
    23476
    NCBI Accession
    NP_055114
    UniProt
    O60885
    Pathways
    Chromatin Binding, SARS-CoV-2 Protein Interactome
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