Metabotropic Glutamate Receptor 6 antibody (AA 700-749)

Catalog No. ABIN6735766

Product Details anti-Metabotropic Glutamate Receptor 6 Antibody

Target: Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))

Binding Specificity: AA 700-749

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GRM6 / MGLUR6

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa700-749 of human GRM6 (O15303, NP_000834). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Opossum (92%), Baboon, Shrew, Dolphin, Pig, Zebra finch, Armadillo, Platypus, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Metabotropic Glutamate Receptor 6

Target: Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))

Alternative Name: GRM6 / MGLUR6 (GRM6 Products)

Background: Name/Gene ID: GRM6
Subfamily: Metabotropic glutamate
Family: GPCR

Synonyms: GRM6, CSNB1B, GPRC1F, MGlu6, MGLUR6

Gene ID: 2916

NCBI Accession: NP_000834

UniProt: O15303