Recombinant Rpb1 CTD antibody (pTyr1)
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- Target See all Rpb1 CTD products
- Rpb1 CTD (RNA Polymerase II CTD)
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- pTyr1
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Rpb1 CTD antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- Brand
- AbFlex®
- Purification
- Protein A Chromatography
- Immunogen
- This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
- Isotype
- IgG2a
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anti-RNA Polymerase II CTD (pSer5) antibody
Reactivity: Human, Mouse WB, ChIP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibodyReactivity: Human, Mouse WB, IF, ICC, ChIP Host: Rabbit Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5), (Ser5) antibodyReactivity: Human, Mouse WB, ChIP, ChIP-seq Host: Rabbit Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer2) antibodyReactivity: Human WB, IF, ICC Host: Rat Monoclonal 3E7C7 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibodyReactivity: Human WB, IF, ICC Host: Rat Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (pSer7) antibodyReactivity: Human WB, IF, ICC Host: Rat Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (pThr4) antibodyReactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibodyReactivity: Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Arabidopsis thaliana, Drosophila melanogaster, Hamster WB, IF, ICC, ELISA, FACS Host: Mouse Monoclonal 8A7 unconjugated
anti-RNA Polymerase II CTD (C-Term) antibodyReactivity: Human, Mouse, Zebrafish (Danio rerio), Dog, Rat, Monkey WB, IF, IHC, IC Host: Rabbit Polyclonal unconjugated
anti-RNA Polymerase II CTD (C-Term), (pSer5) antibodyReactivity: Human, Mouse, Zebrafish (Danio rerio), Dog, Rat WB, IF, IHC, IC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Validated Applications:
IF: 0.5 - 2 µg/ml
Bead-based ELISA: 0.5 - 10 µg/ml
WB: 1 - 2 μg/mL
For optimal results, we recommend the addition of 0.1% Tween 20 to all Blotto solutions to reduce background. Individual optimization may be required. - Comment
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AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- Rpb1 CTD (RNA Polymerase II CTD)
- Alternative Name
- RNA Pol II CTD (Rpb1 CTD Products)
- Molecular Weight
- 210 kDa
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