IL12A antibody (AA 51-150)

Catalog No. ABIN672951

Product Details anti-IL12A Antibody

Target: IL12A (Interleukin 12 alpha (IL12A))

Binding Specificity: AA 51-150

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse IL-12 alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-IL12A antibody (ABIN672951)

Yang, Liu, Zhang, Hu, Chen, Wang, Yin, Li, Qi: "Effect of IL-17 in the development of colon cancer in mice." in: Oncology letters, Vol. 12, Issue 6, pp. 4929-4936, (2017) (PubMed).

Taylor-Fishwick, Weaver, Grzesik, Chakrabarti, Green-Mitchell, Imai, Kuhn, Nadler: "Production and function of IL-12 in islets and beta cells." in: Diabetologia, Vol. 56, Issue 1, pp. 126-35, (2012) (PubMed).

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: IL-12 alpha (IL12A Products)

Background:

Synonyms: p35, Ll12a, Il-12a, IL-12p35, Interleukin-12 subunit alpha, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, Il12a

Background: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Gene ID: 16159

UniProt: P43431

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation