Vimentin antibody (AA 371-466)

Catalog No. ABIN672786

Product Details anti-Vimentin Antibody

Target: Vimentin (VIM)

Binding Specificity: AA 371-466

Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Specificity: KO-Validated

Cross-Reactivity: Cow, Human, Monkey, Mouse, Pig, Rat

Predicted Reactivity: Cow,Chicken,Goat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Vimentin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Vimentin antibody (ABIN672786)

Pagani, Ghezzi, Ulisse, Rubio, Turrini, Garavaglia, Candiani, Castilletti, Ippolito, Poli, Broccoli, Panina-Bordignon, Vicenzi: "Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus." in: Scientific reports, Vol. 7, pp. 44286, (2018) (PubMed).

Smith, Zhang, Schiele, Khademhosseini, Kuo, Yelick: "Developing a biomimetic tooth bud model." in: Journal of tissue engineering and regenerative medicine, (2017) (PubMed).

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, (2017) (PubMed).

Tanos, Saibene, Pipolo, Battaglia, Felisati, Rubio: "Isolation of putative stem cells present in human adult olfactory mucosa." in: PLoS ONE, Vol. 12, Issue 7, pp. e0181151, (2017) (PubMed).

Park, Sung, Jeong, Choi, Lee, Kwon, Jang, Park, Kim, Kim, Yoon, Ryoo, Kim: "Critical roles of hMAGEA2 in induced pluripotent stem cell pluripotency, proliferation, and differentiation." in: Cell biochemistry and function, Vol. 35, Issue 7, pp. 392-400, (2017) (PubMed).

Wang, Cao, Liu, Du, Wang, Chen, Liu, Wu: "5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 46-56, (2017) (PubMed).

Djamali, Wilson, Sadowski, Zha, Niles, Hafez, Dorn, Mehner, Grimm, Hoffmann, Zhong, Fain, Reese: "Nox2 and Cyclosporine-Induced Renal Hypoxia." in: Transplantation, Vol. 100, Issue 6, pp. 1198-210, (2017) (PubMed).

Fagundes, Fernandes, Paraense, de Farias-Junior, Teixeira, Alves-Junior, Pinheiro, Crespo-López, Maia, Lima: "Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 7323627, (2017) (PubMed).

Yap, Digilio, McMahon, Roszkowska, Bott, Kruczek, Winckler: "Different Doublecortin (DCX) Patient Alleles Show Distinct Phenotypes in Cultured Neurons: EVIDENCE FOR DIVERGENT LOSS-OF-FUNCTION AND "OFF-PATHWAY" CELLULAR MECHANISMS." in: The Journal of biological chemistry, Vol. 291, Issue 52, pp. 26613-26626, (2017) (PubMed).

Khayat, Monteiro, Smith, Pagni, Zhang, Khademhosseini, Yelick: "GelMA-Encapsulated hDPSCs and HUVECs for Dental Pulp Regeneration." in: Journal of dental research, Vol. 96, Issue 2, pp. 192-199, (2017) (PubMed).

Wang, Nikhil, Viccaro, Chang, Jacobsen, Sandusky, Shah: "The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma." in: Journal of cell science, Vol. 130, Issue 6, pp. 1078-1093, (2017) (PubMed).

Wang, Jin, Fan, Wang, Xing, Niu: "Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172233, (2017) (PubMed).

Sucre, Wilkinson, Vijayaraj, Paul, Dunn, Alva-Ornelas, Gomperts: "A three-dimensional human model of the fibroblast activation that accompanies bronchopulmonary dysplasia identifies Notch-mediated pathophysiology." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 310, Issue 10, pp. L889-98, (2016) (PubMed).

Huang, Wen: "Phenotype transformation of immortalized NCM460 colon epithelial cell line by TGF-β1 is associated with chromosome instability." in: Molecular biology reports, Vol. 43, Issue 10, pp. 1069-78, (2016) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin (VIM Products)

Synonyms: CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background:

Synonyms: HEL113, CTRCT3, Vimentin, VIM

Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis