His Tag antibody (DyLight 800)

Catalog No. ABIN6720719

Supplier Product No.: 200-345-382

Product Details anti-His Tag Antibody (DyLight 800)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to DyLight 800

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Supplier Product No.: 200-345-382

Supplier: Rockland

Purpose: 6X His Tag Antibody Dylight™ 800 Conjugated

Cross-Reactivity (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Purification: This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Clone: 33D10-D2-G8

Isotype: IgG1 kappa

Labeling Ratio: 2.6

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000-1:25,000

Other: User Optimized

Comment:

Mouse Anti-6X HIS EPITOPE TAG DyLight™800 Conjugated has been tested in ELISA, dot blot, and western blot and is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-His Tag antibody (DyLight 800) (ABIN6720719)

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Target Details for His Tag

Target: His Tag

Abstract: His Tag Products

Background:

Synonyms: mouse anti-6X His Tag DyLight™ 800 conjugated Antibody, DyLight™ 800 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.