His Tag antibody (DyLight 405)

Catalog No. ABIN6720710

Supplier Product No.: 200-346-382

Product Details anti-His Tag Antibody (DyLight 405)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to DyLight 405

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 200-346-382

Supplier: Rockland

Purpose: 6X His Tag Antibody Dylight™ 405 Conjugated

Cross-Reactivity (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Purification: 6X His Tag Antibody is directed against the 6X His motif and is useful in determining its presence in various assays.

Immunogen: This 6X HIS antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Clone: 33D10-D2-G8

Isotype: IgG1 kappa

Labeling Ratio: 2.4

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000-1:25,000

Comment:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store 6X His Tag Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Abstract: His Tag Products

Background:

Synonyms: mouse anti-6X His Tag biotin conjugated Antibody, biotin conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: This antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.