GFP antibody (DyLight 488)

Catalog No. ABIN6720701

Supplier Product No.: 600-141-215

Product Details anti-GFP Antibody (DyLight 488)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to DyLight 488

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA, Dot Blot (DB)

Supplier Product No.: 600-141-215

Supplier: Rockland

Purpose: GFP Antibody Dylight™ 488 Conjugated

Specificity: Wt, rGFP, eGFP

Cross-Reactivity (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Purification: GFP Dylight™ 488 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Isotype: IgG

Labeling Ratio: 5.7

Application Details

Application Notes:

FLISA_Dilution: >1:10,000

ELISA_Dilution: 1:20,000 - 1:40,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000

Other: User Optimized

Comment:

Suggested Applications: FC, IF, IHC, IP, Multiplex
Anti-GFP Dylight™ 488 Conjugated Antibody has been tested by dot blot and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Conjugated anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (DyLight 488) (ABIN6720701)

Gentili, Lahaye, Nadalin, Nader, Puig Lombardi, Herve, De Silva, Rookhuizen, Zueva, Goudot, Maurin, Bochnakian, Amigorena, Piel, Fachinetti, Londoño-Vallejo, Manel: "The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus." in: Cell reports, Vol. 26, Issue 9, pp. 2377-2393.e13, (2019) (PubMed).

Haas, Meiler, Geiger, Vogt, Preuss, Kochs, Pichlmair: "Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity." in: PLoS pathogens, Vol. 14, Issue 4, pp. e1006980, (2018) (PubMed).

Begolly, Olschowka, Love, Williams, OBanion: "Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion." in: Glia, Vol. 66, Issue 4, pp. 846-861, (2018) (PubMed).

Dufour, Gillet, Frankel, Weibel, Emonet: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).

Chang, Kunasegaran, Tarulli, De Silva, Voorhoeve, Pietersen: "New insights into lineage restriction of mammary gland epithelium using parity-identified mammary epithelial cells." in: Breast cancer research : BCR, Vol. 16, Issue 1, pp. R1, (2015) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background:

Synonyms: goat anti-GFP Antibody DyLight™ 488 Conjugation, DyLight™ 488 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP Dylight™ 488 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P42212