GFP antibody (DyLight 488)

Catalog No. ABIN6720701

Supplier Product No.: 600-141-215

Product Details anti-GFP Antibody (DyLight 488)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to DyLight 488

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Supplier Product No.: 600-141-215

Supplier: Rockland

Purpose: GFP Antibody Dylight™ 488 Conjugated

Specificity: Wt, rGFP, eGFP

Cross-Reactivity (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Purification: GFP Dylight™ 488 Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Isotype: IgG

Labeling Ratio: 5.7

Application Details

Application Notes:

FLISA_Dilution: >1:10,000

ELISA_Dilution: 1:20,000 - 1:40,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000

Other: User Optimized

Comment:

Suggested Applications: FC, IF, IHC, IP, Multiplex
Anti-GFP Dylight™ 488 Conjugated Antibody has been tested by dot blot and western blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Conjugated anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (DyLight 488) (ABIN6720701)

Park, Kim, Alabdalla, Mishra, Mchaourab: "Generation and characterization of a zebrafish knockout model of abcb4, a homolog of the human multidrug efflux transporter P-glycoprotein." in: Human genomics, Vol. 17, Issue 1, pp. 84, (2023) (PubMed).

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Kim, Leng, Park, Sorets, Kim, Shostak, Embalabala, Mlouk, Katdare, Rose, Sturgeon, Neal, Ao, Wang, Sofroniew, Brunger, McMahon, Schrag, Kampmann, Lippmann: "Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin." in: Nature communications, Vol. 13, Issue 1, pp. 6581, (2022) (PubMed).

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Bhargava, Williart, Maurin, Davidson, Jouve, Piel, Lahaye, Manel: "Inhibition of HIV infection by structural proteins of the inner nuclear membrane is associated with reduced chromatin dynamics." in: Cell reports, Vol. 36, Issue 13, pp. 109763, (2022) (PubMed).

Lee, Zhao, Wong, Chrysostomou, Crowston, Bui: "The Effect of Aging on Retinal Function and Retinal Ganglion Cell Morphology Following Intraocular Pressure Elevation." in: Frontiers in aging neuroscience, Vol. 14, pp. 859265, (2022) (PubMed).

Wang, Wong, Lee, Bui, Dudczig, Vessey, Fletcher: "Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout." in: Scientific reports, Vol. 11, Issue 1, pp. 4184, (2021) (PubMed).

Freter, Falletta, Omrani, Rasa, Herbert, Annunziata, Minetti, Krepelova, Adam, Käppel, Rüdiger, Wang, Goding, Neri: "Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells." in: Nature communications, Vol. 12, Issue 1, pp. 3318, (2021) (PubMed).

Xu, Brawner, Li, Liu, Kim, Xue, Pang, Kim, Hart, Liu, Jiang: "OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome." in: Cell stem cell, Vol. 24, Issue 6, pp. 908-926.e8, (2020) (PubMed).

Russo, Latour, McGavern: "Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury." in: Nature immunology, Vol. 19, Issue 5, pp. 442-452, (2019) (PubMed).

Fletcher, Greferath, Saha, Anderson, Vessey: "Ganglion Cell Assessment in Rodents with Retinal Degeneration." in: Methods in molecular biology (Clifton, N.J.), Vol. 1753, pp. 261-273, (2019) (PubMed).

Gentili, Lahaye, Nadalin, Nader, Puig Lombardi, Herve, De Silva, Rookhuizen, Zueva, Goudot, Maurin, Bochnakian, Amigorena, Piel, Fachinetti, Londoño-Vallejo, Manel: "The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus." in: Cell reports, Vol. 26, Issue 9, pp. 2377-2393.e13, (2019) (PubMed).

Kolter, Feuerstein, Zeis, Hagemeyer, Paterson, dErrico, Baasch, Amann, Masuda, Lösslein, Gharun, Meyer-Luehmann, Waskow, Franzke, Grün, Lämmermann, Prinz, Henneke: "A Subset of Skin Macrophages Contributes to the Surveillance and Regeneration of Local Nerves." in: Immunity, Vol. 50, Issue 6, pp. 1482-1497.e7, (2019) (PubMed).

Haas, Meiler, Geiger, Vogt, Preuss, Kochs, Pichlmair: "Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity." in: PLoS pathogens, Vol. 14, Issue 4, pp. e1006980, (2018) (PubMed).

Begolly, Olschowka, Love, Williams, OBanion: "Fractionation enhances acute oligodendrocyte progenitor cell radiation sensitivity and leads to long term depletion." in: Glia, Vol. 66, Issue 4, pp. 846-861, (2018) (PubMed).

Bongianino, Denegri, Mazzanti, Lodola, Vollero, Boncompagni, Fasciano, Rizzo, Mangione, Barbaro, Di Fonso, Napolitano, Auricchio, Protasi, Priori: "Allele-Specific Silencing of Mutant mRNA Rescues Ultrastructural and Arrhythmic Phenotype in Mice Carriers of the R4496C Mutation in the Ryanodine Receptor Gene (RYR2)." in: Circulation research, Vol. 121, Issue 5, pp. 525-536, (2017) (PubMed).

Dufour, Gillet, Frankel, Weibel, Emonet: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).

"Correction: Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005149, (2016) (PubMed).

Ho, Vessey, Fletcher: "Immunolocalization of the P2X4 receptor on neurons and glia in the mammalian retina." in: Neuroscience, Vol. 277, pp. 55-71, (2015) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background:

Synonyms: goat anti-GFP Antibody DyLight™ 488 Conjugation, DyLight™ 488 conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP Dylight™ 488 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P42212