Biotin antibody (DyLight 488)

Catalog No. ABIN6720659

Product Details anti-Biotin Antibody (DyLight 488)

Target: Biotin

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to DyLight 488

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Immunogen Type: Other

Isotype: IgG

Labeling Ratio: 4.0

Application Details

Application Notes:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

References for anti-Biotin antibody (DyLight 488) (ABIN6720659)

Lipovšek, Carvajal, Allentoff, Barros, Brailsford, Cong, Cotter, Gangwar, Hollander, Lafont, Lau, Li, Moreta, ONeil, Pinckney, Smith, Su, Terragni, Wallace, Wang, Wright, Marsh, Bryson: "Adnectin-drug conjugates for Glypican-3-specific delivery of a cytotoxic payload to tumors." in: Protein engineering, design & selection : PEDS, Vol. 31, Issue 5, pp. 159-171, (2019) (PubMed).

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background:

Synonyms: Goat anti Biotin Antibody Dylight™ 488 Conjugated, Goat anti-biotin Antibody Dylight™ 488 Conjugated

Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin DyLight Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.