His Tag antibody (DyLight 649)

Catalog No. ABIN6720637

Product Details anti-His Tag Antibody (DyLight 649)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to DyLight 649

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Purification: This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.  To date, this antibody has reacted with all His tagged proteins so far tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.

Immunogen:

Immunogen: This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Immunogen Type: Peptide

Clone: 33D10-D2

Isotype: IgG1

Labeling Ratio: 2.5

Application Details

Application Notes:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000-1:25,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

References for anti-His Tag antibody (DyLight 649) (ABIN6720637)

Kariolis, Miao, Diep, Nash, Olcina, Jiang, Jones, Kapur, Mathews, Koong, Rankin, Cochran, Giaccia: "Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies." in: The Journal of clinical investigation, Vol. 127, Issue 1, pp. 183-198, (2017) (PubMed).

Kariolis, Miao, Jones, Kapur, Mathews, Giaccia, Cochran: "An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis." in: Nature chemical biology, Vol. 10, Issue 11, pp. 977-83, (2014) (PubMed).

Target Details for His Tag

Target: His Tag

Alternative Name: 6X HIS tagged (His Tag Products)

Target Type: Tag

Background:

Synonyms: mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Background: Anti 6X HIS tagged Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.