TRAC antibody (AA 1-114)
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- Target See all TRAC products
- TRAC (T Cell Receptor alpha Constant (TRAC))
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Binding Specificity
- AA 1-114
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TRAC antibody is un-conjugated
- Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS)
- Purpose
- Rabbit IgG polyclonal antibody for TCR alpha detection. Tested with WB, FCM, Direct ELISA in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Rabbit IgG polyclonal antibody for TCR alpha detection. Tested with WB, FCM, Direct ELISA in Human,Mouse,Rat.
- Purification
- Immunogen affinity purified.
- Immunogen
- E. coli-derived human TCR alpha recombinant protein (Position: I1-L114).
- Isotype
- IgG
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- Application Notes
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Application details: Western blot|0.1-0.5 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL
- Comment
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Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- TRAC (T Cell Receptor alpha Constant (TRAC))
- Alternative Name
- TRAC (TRAC Products)
- Synonyms
- TCRA antibody, Gm16914 antibody, Tcra antibody, Tcra-C antibody, T-cell receptor alpha constant antibody, T cell receptor alpha constant antibody, TRAC antibody, Trac antibody
- Background
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Synonyms: T-cell receptor alpha chain C region, TRAC, TCRA
Background: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.
- Gene ID
- 28755
- UniProt
- P01848
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