Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4) (AA 10-459) antibody

Catalog No. ABIN6719648

Product Details

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Binding Specificity: AA 10-459

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit IgG polyclonal antibody for MT-ND4 detection. Tested with WB, Direct ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MT-ND4 detection. Tested with WB, Direct ELISA in Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details

Target: Mitochondrially Encoded NADH Dehydrogenase 4 (MT-ND4)

Alternative Name: MT-ND4 (MT-ND4 Products)

Synonyms: MTND4 antibody, NADH4 antibody, NADH dehydrogenase, subunit 4 (complex I) antibody, NADH dehydrogenase subunit 4 antibody, NADH dehydrogenasesubunit 4 antibody, ND4 antibody, nad4 antibody, NAD4 antibody

Background:

Synonyms: NADH-ubiquinone oxidoreductase chain 4, NADH dehydrogenase subunit 4, MT-ND4, MTND4, NADH4, ND4,

Background: NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial geneMT-ND4. MT-ND4 is a subunit of the respiratory chain Complex I that is believed to belong to the minimal assembly of core proteins required to catalyze NADH dehydrogenation and electron transfer to ubiquinone (coenzyme Q10). Initially, NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.

Gene ID: 4538

UniProt: P03905