PPCS antibody

Catalog No. ABIN6719631

Product Details anti-PPCS Antibody

Target: PPCS (Phosphopantothenoylcysteine Synthetase (PPCS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPCS antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purpose: Rabbit IgG polyclonal antibody for PPCS detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human,Mouse,Rat.

Sequence: VEIEEKIVDN LQSRHTAFIG DRN

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for PPCS detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence of human PPCS(VEIEEKIVDNLQSRHTAFIGDRN).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunohistochemistry(Frozen Section)|0.5-1 μg/mL Immunocytochemistry|0.5-1 μg/mL Flow Cytometry|1-3 μg/1x106 cells

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for PPCS

Target: PPCS (Phosphopantothenoylcysteine Synthetase (PPCS))

Alternative Name: PPCS (PPCS Products)

Synonyms: RGD1311399 antibody, BcDNA:GH03502 antibody, CG5629 antibody, Dmel\\CG5629 antibody, GH03502 antibody, PPCS antibody, anon-WO0118547.401 antibody, dPPCS antibody, GB14583 antibody, MGC80633 antibody, im:7147515 antibody, zgc:136566 antibody, DFP antibody, 6330579B17Rik antibody, AI413471 antibody, AI790434 antibody, phosphopantothenoylcysteine synthetase antibody, Phosphopantothenoylcysteine synthetase antibody, phosphopantothenate--cysteine ligase antibody, phosphopantothenoylcysteine synthetase L homeolog antibody, Ppcs antibody, LOC411581 antibody, ppcs.L antibody, PPCS antibody, ppcs antibody

Background:

Synonyms: Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB

Background: Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene. Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. By genomic sequence analysis, this PPCS gene ia mapped to chromosome 1.

Gene ID: 79717

Pathways: Ribonucleoside Biosynthetic Process