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BCL9L antibody (AA 397-666)

BCL9L Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6719451
  • Target See all BCL9L Antibodies
    BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))
    Binding Specificity
    • 13
    • 10
    • 7
    • 1
    • 1
    • 1
    AA 397-666
    Reactivity
    Human, Mouse, Rat
    Host
    • 26
    • 1
    Rabbit
    Clonality
    • 26
    • 1
    Polyclonal
    Conjugate
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BCL9L antibody is un-conjugated
    Application
    • 20
    • 16
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human BCL9L recombinant protein (Position: E397-A666).
    Isotype
    IgG
    Top Product
    Discover our top product BCL9L Primary Antibody
  • Application Notes

    Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

    Comment

    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Target
    BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))
    Alternative Name
    BCL9L (BCL9L Products)
    Synonyms
    BCL9-2 antibody, DLNB11 antibody, B9L antibody, BC003321 antibody, bcl9-2 antibody, BCL9L antibody, B-cell CLL/lymphoma 9 like antibody, B cell CLL/lymphoma 9-like antibody, B-cell CLL/lymphoma 9-like antibody, BCL9L antibody, Bcl9l antibody, bcl9l antibody
    Background

    Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2, BCL9L, DLNB11

    Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9, also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514-517 and 970-973), and one poly-Ala segment (aa 900-903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391-1426. Over aa 1009-1328, human Bcl-9 is 96 % aa identical to mouse Bcl-9.

    Gene ID
    283149
    Pathways
    Stem Cell Maintenance
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