BCL9L antibody (AA 397-666)
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- Target See all BCL9L Antibodies
- BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))
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Binding Specificity
- AA 397-666
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BCL9L antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Rabbit IgG polyclonal antibody for BCL9L detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.
- Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human BCL9L recombinant protein (Position: E397-A666).
- Isotype
- IgG
- Top Product
- Discover our top product BCL9L Primary Antibody
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- Application Notes
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Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL
- Comment
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))
- Alternative Name
- BCL9L (BCL9L Products)
- Synonyms
- BCL9-2 antibody, DLNB11 antibody, B9L antibody, BC003321 antibody, bcl9-2 antibody, BCL9L antibody, B-cell CLL/lymphoma 9 like antibody, B cell CLL/lymphoma 9-like antibody, B-cell CLL/lymphoma 9-like antibody, BCL9L antibody, Bcl9l antibody, bcl9l antibody
- Background
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Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, Protein BCL9-2, BCL9L, DLNB11
Background: B-cell CLL/lymphoma 9 like is a protein that in humans is encoded by the BCL9L gene. It is mapped to 11q23.3. Bcl-9 (B-cell lymphoma 9, also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514-517 and 970-973), and one poly-Ala segment (aa 900-903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391-1426. Over aa 1009-1328, human Bcl-9 is 96 % aa identical to mouse Bcl-9.
- Gene ID
- 283149
- Pathways
- Stem Cell Maintenance
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