MED15 antibody (AA 1-285)

Catalog No. ABIN6719342

Product Details anti-MED15 Antibody

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Binding Specificity: AA 1-285

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Mouse IgG monoclonal antibody for MED15 detection. Tested with WB, ICC/IF, FCM in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Mouse IgG monoclonal antibody for MED15 detection. Tested with WB, ICC/IF, FCM in Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MED15 recombinant protein (Position: M1-A285).

Clone: 6F4

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunocytochemistry/Immunofluorescence|2 μg/mL Flow Cytometry|1-3 μg/1x106 cells

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15 (MED15 Products)

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine, Q-rich-associated protein, PC2 glutamine, Q-rich-associated protein, TPA-inducible gene 1 protein, TIG-1, Trinucleotide repeat-containing gene 7 protein, MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7

Background: Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.

Gene ID: 51586

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha