Fibronectin antibody (AA 1201-1300)

Catalog No. ABIN671646

Product Details anti-Fibronectin Antibody

Target: Fibronectin

Binding Specificity: AA 1201-1300

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Fibronectin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Fibronectin antibody (ABIN671646)

Miyauchi, Yasuchika, Fukumitsu, Ishii, Ogiso, Minami, Kojima, Yamaoka, Katayama, Kawai, Yoshitoshi-Uebayashi, Kita, Yasuda, Sasaki, Uemoto: "A novel three-dimensional culture system maintaining the physiological extracellular matrix of fibrotic model livers accelerates progression of hepatocellular carcinoma cells." in: Scientific reports, Vol. 7, Issue 1, pp. 9827, (2017) (PubMed).

Yang, Li, Qi, Li, Zhou, Qing, Zhang, Gao: "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." in: PeerJ, Vol. 5, pp. e3950, (2017) (PubMed).

Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, (2017) (PubMed).

Elmas, Erbas, Yigitturk: "The efficacy of Aesculus hippocastanum seeds on diabetic nephropathy in a streptozotocin-induced diabetic rat model." in: Biomedicine & pharmacotherapy, Vol. 83, pp. 392-396, (2016) (PubMed).

Kang, Baik, Yoo, Kim, Cheong, Park, Kang, Ha: "Aliskiren Regulates Neonatal Fc Receptor and IgG Metabolism with Attenuation of Anti-GBM Glomerulonephritis in Mice." in: Nephron, Vol. 134, Issue 4, pp. 272-282, (2016) (PubMed).

Ji, Wu, Ma, Ma, Qin: "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." in: Molecular biology reports, Vol. 41, Issue 4, pp. 2151-9, (2014) (PubMed).

Karadeniz, Cavuso?lu, Turkmen, Uyan?kgil, Karadeniz, Akdemir, Tuglu, Ates, Erbas: "Experimental comparison of protective characteristics of enalapril and trimetazidine in diabetic nephropathy." in: Renal failure, Vol. 36, Issue 8, pp. 1283-90, (2014) (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

Wu, Zhang, Ma, Zhang, Qin: "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." in: Molecular biology reports, Vol. 39, Issue 9, pp. 9085-93, (2012) (PubMed).

Bai, Zheng, Fang, Wang, Hou, Li, Chen, Tian: "Fabrication of engineered heart tissue grafts from alginate/collagen barium composite microbeads." in: Biomedical materials (Bristol, England), Vol. 6, Issue 4, pp. 45002, (2011) (PubMed).

Xu, Chen, Huang, Varghese, Moorhead, Yan, Powis, Li, Ruan: "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." in: American journal of physiology. Renal physiology, Vol. 301, Issue 4, pp. F713-22, (2011) (PubMed).

Ma, Tao, Lu, Jiang: "Intraocular expression of serum amyloid a and interleukin-6 in proliferative diabetic retinopathy." in: American journal of ophthalmology, Vol. 152, Issue 4, pp. 678-685.e2, (2011) (PubMed).

Jun, Song, Chen, Zanhua, Yonghong, Shuxia, Huijun: "In vivo and in vitro effects of SREBP-1 on diabetic renal tubular lipid accumulation and RNAi-mediated gene silencing study." in: Histochemistry and cell biology, Vol. 131, Issue 3, pp. 327-45, (2009) (PubMed).

Target Details for Fibronectin

Target: Fibronectin

Abstract: Fibronectin Products

Synonyms: FN antibody, cig antibody, fibronectin antibody, finc antibody, lets antibody, msf antibody, CIG antibody, ED-B antibody, FINC antibody, FNZ antibody, GFND antibody, GFND2 antibody, LETS antibody, MSF antibody, E330027I09 antibody, Fn antibody, Fn-1 antibody, FIBNEC antibody, fn-1 antibody, fibronectin 1 antibody, FN1 antibody, fn1 antibody, Fn1 antibody

Background:

Synonyms: Fn, Fn-1, E3327I9, Fibronectin, Fn1

Background: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling (By similarity).

Gene ID: 14268

UniProt: P11276