HMGB1 antibody (AA 75-170)

Catalog No. ABIN671616

Product Details anti-HMGB1 Antibody

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Binding Specificity: AA 75-170

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HMGB1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HMGB1 antibody (ABIN671616)

Li, Xu, Xu, Cai, Sun, Yang, Sun, Lu: "Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression." in: Molecular and cellular biochemistry, Vol. 420, Issue 1-2, pp. 161-70, (2016) (PubMed).

Li, Jia, Ji, Gong, Wang, Zhang, Wang, Zang: "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." in: International journal of molecular medicine, Vol. 38, Issue 3, pp. 767-75, (2016) (PubMed).

Sun, Zhang, Cui, Chen, Xie, Zhou, Liu, Peng, Zhang: "High mobility group box-1 and its clinical value in breast cancer." in: OncoTargets and therapy, Vol. 8, pp. 413-9, (2015) (PubMed).

Zhang, He, Wang, Zhou, Ma: "Omega-3 polyunsaturated fatty acids inhibit the increase in cytokines and chemotactic factors induced in vitro by lymph fluid from an intestinal ischemia-reperfusion injury model." in: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 31, Issue 3, pp. 508-14, (2015) (PubMed).

Luan, Kan, Xu, Lv, Jiang: "Rosmarinic acid protects against experimental diabetes with cerebral ischemia: relation to inflammation response." in: Journal of neuroinflammation, Vol. 10, pp. 28, (2013) (PubMed).

Zhao, Hu, Sun, Sun: "The high mobility group box 1 protein of Sciaenops ocellatus is a secreted cytokine that stimulates macrophage activation." in: Developmental and comparative immunology, Vol. 35, Issue 10, pp. 1052-8, (2011) (PubMed).

Jiang, Fu, Tian, Zhu, Hou: "Curculigoside A attenuates experimental cerebral ischemia injury in vitro and vivo." in: Neuroscience, Vol. 192, pp. 572-9, (2011) (PubMed).

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1 (HMGB1 Products)

Synonyms: HMG1 antibody, HMG3 antibody, SBP-1 antibody, DEF antibody, HMG-1 antibody, Hmg1 antibody, amphoterin antibody, p30 antibody, hmgb1 antibody, ik:tdsubc_1a5 antibody, wu:fb23c02 antibody, xx:tdsubc_1a5 antibody, zgc:56110 antibody, zgc:77104 antibody, hmg-1 antibody, hmg3 antibody, sbp-1 antibody, hmg1 antibody, HMGB1 antibody, Ac2-008 antibody, high mobility group box 1 antibody, high-mobility group box 1 antibody, high mobility group box 1a antibody, high mobility group box 1 L homeolog antibody, high mobility group protein B1 antibody, HMGB1 antibody, Hmgb1 antibody, hmgb1 antibody, hmgb1a antibody, hmgb1.L antibody, LOC100359149 antibody

Background:

Synonyms: HMG1, HMG3, SBP-1, High mobility group protein B1, High mobility group protein 1, HMG-1, HMGB1

Background: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome