CD8 antibody (AA 51-150)

Catalog No. ABIN671391

Product Details anti-CD8 Antibody

Key Features:

• High quality CD8 Primary Antibody for the detection of CD8.
• Reliable product with high standard validation data

Target: CD8

Binding Specificity: AA 51-150

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD8

Isotype: IgG

Product Specific Information:

What can the CD8 Antibody ABIN671391 be used for?: This polyclonal CD8 Antibody detects CD8. The CD8 Antibody has been validated for various applications and can be used for the detection of CD8 and derivatives by Flow Cytometry, Immunohistochemistry (Frozen Sections), Western Blotting, ELISA, Immunohistochemistry (Paraffin-embedded Sections), Immunofluorescence (Cultured Cells), Immunofluorescence (Paraffin-embedded Sections).

What validation data is available for this CD8 Antibody?: The primary antibody is referenced in 23 publications and is characterized by a proven, very high reliability. It has currently 7 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. CD8 Antibody for the detection of CD8 and derivatives.

What is the function of CD8?: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD8 antibody (ABIN671391)

Kovtun, Bergdolt, Hägele, Matthes, Lambris, Huber-Lang, Ignatius: "Complement receptors C5aR1 and C5aR2 act differentially during the early immune response after bone fracture but are similarly involved in bone repair." in: Scientific reports, Vol. 7, Issue 1, pp. 14061, (2019) (PubMed).

Wang, Sun, Zhang, Hu, Sun, Sheng, Fu: "Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway." in: American journal of translational research, Vol. 10, Issue 1, pp. 114-125, (2018) (PubMed).

Rapp, Hachemi, Kemmler, Koenen, Tuckermann, Ignatius: "Induced global deletion of glucocorticoid receptor impairs fracture healing." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 4, pp. 2235-2245, (2018) (PubMed).

Schmid, Bouazzaoui, Schmid, Birner, Schach, Maier, Holler, Endemann: "Acute Renal Graft-Versus-Host Disease in a Murine Model of Allogeneic Bone Marrow Transplantation." in: Cell transplantation, Vol. 26, Issue 8, pp. 1428-1440, (2018) (PubMed).

Chuang, Jan, Chang, Lu: "The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 210, (2017) (PubMed).

Wang, Johnson, He, Rong, Wong, Nigam, Behfar, Xu, Christman: "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." in: Biomaterials, Vol. 129, pp. 98-110, (2017) (PubMed).

Willett, Liu, Berke, Giannini, Schmid, Jia, Wang, Wang, Samson, Yu, Wang, Nawshad, Reinhardt: "Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing." in: Journal of periodontology, Vol. 88, Issue 8, pp. 799-807, (2017) (PubMed).

Haffner-Luntzer, Fischer, Prystaz, Liedert, Ignatius: "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." in: European journal of medical research, Vol. 22, Issue 1, pp. 23, (2017) (PubMed).

Kroner, Kovtun, Kemmler, Messmann, Strauss, Seitz, Schinke, Amling, Kotrba, Froebel, Dudeck, Dudeck, Ignatius: "Mast Cells Are Critical Regulators of Bone Fracture-Induced Inflammation and Osteoclast Formation and Activity." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 32, Issue 12, pp. 2431-2444, (2017) (PubMed).

Tanaka, Goshima, Esaki, Sato, Murata, Nishiyama, Watanabe, Kimura: "The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model." in: American journal of cancer research, Vol. 7, Issue 8, pp. 1693-1703, (2017) (PubMed).

Prystaz, Kaiser, Kovtun, Haffner-Luntzer, Fischer, Rapp, Liedert, Strauss, Waetzig, Rose-John, Ignatius: "Distinct Effects of IL-6 Classic and Trans-Signaling in Bone Fracture Healing." in: The American journal of pathology, Vol. 188, Issue 2, pp. 474-490, (2017) (PubMed).

Jaime-Ramirez, Yu, Caserta, Yoo, Zhang, Lee, Hofmeister, Lee, Kumar, Pan, Kumar, Baiocchi, Teknos, Pichiorri, Kaur, Old: "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." in: Molecular therapy oncolytics, Vol. 5, pp. 87-96, (2017) (PubMed).

Ashraf, Schwelberger, Brendel, Feurle, Andrassy, Kotsch, Regele, Pratschke, Maier, Aigner: "Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 16, Issue 3, pp. 808-20, (2016) (PubMed).

Kovtun, Bergdolt, Wiegner, Radermacher, Huber-Lang, Ignatius: "The crucial role of neutrophil granulocytes in bone fracture healing." in: European cells & materials, Vol. 32, pp. 152-62, (2016) (PubMed).

Li, Kobayashi, Mona, Satomi, Okano, Inoue, Tani, Takahashi: "Immunogenic FEAT protein circulates in the bloodstream of cancer patients." in: Journal of translational medicine, Vol. 14, Issue 1, pp. 275, (2016) (PubMed).

Ju, Xing, Yang, Zheng, Jia, Zhang, Ren: "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." in: BMC immunology, Vol. 17, Issue 1, pp. 31, (2016) (PubMed).

Wang, Quan, Yan, Morales, Wetsel: "Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells." in: Stem cells translational medicine, Vol. 4, Issue 10, pp. 1234-45, (2015) (PubMed).

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Synonyms: CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background:

Synonyms: Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Lyt2

Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gene ID: 12525

UniProt: P01731