Cyclin D1 antibody (AA 61-110)

Catalog No. ABIN671016

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Binding Specificity: AA 61-110

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Cow, Human, Mouse, Rabbit, Rat, Sheep

Predicted Reactivity: Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse Cyclin D1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin D1 antibody (ABIN671016)

Jiang, Xie, Cai, Ren, Ma: "Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway." in: BMC microbiology, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).

Wang, Cao, Sun, Dai, Fang, Li, Yan, Mao, Zhang: "Total flavonoid aglycones extract in Radix scutellariae inhibits lung carcinoma and lung metastasis by affecting cell cycle and DNA synthesis." in: Journal of ethnopharmacology, Vol. 194, pp. 269-279, (2016) (PubMed).

Zhang, Pan, Xu, Niu, Ma, Xu: "Interleukin 18 augments growth ability via NF-?B and p38/ATF2 pathways by targeting cyclin B1, cyclin B2, cyclin A2, and Bcl-2 in BRL-3A rat liver cells." in: Gene, Vol. 563, Issue 1, pp. 45-51, (2015) (PubMed).

Wang, Yang, Liu, Huang, Wang, Chen, Chen: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).

Yang, Wang, Hui, Li, Jiang: "SOX 1, contrary to SOX 2, suppresses proliferation, migration, and invasion in human laryngeal squamous cell carcinoma by inhibiting the Wnt/?-catenin pathway." in: Tumour biology, (2015) (PubMed).

Shi, Lu, Zhou, Tan, He, Ding, Tian, Wang, Wang: "Long non-coding RNA Loc554202 regulates proliferation and migration in breast cancer cells." in: Biochemical and biophysical research communications, Vol. 446, Issue 2, pp. 448-53, (2014) (PubMed).

Ye, Li, Li, Yang, Niu, Wang: "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." in: Journal of ethnopharmacology, Vol. 153, Issue 1, pp. 258-67, (2014) (PubMed).

Lin, Wang, Wang, Fang, Zhang, Yang, Guo, Lin, Zhang, Wang: "Lithocarpus polystachyus REHD leaf aqueous extract inhibits human breast cancer growth in vitro and in vivo." in: Nutrition and cancer, Vol. 66, Issue 4, pp. 613-24, (2014) (PubMed).

He, Xiang, Zong, Yan, Yu, Liu, Zou, Yang: "CDK2-AP1 inhibits growth of breast cancer cells by regulating cell cycle and increasing docetaxel sensitivity in vivo and in vitro." in: Cancer cell international, Vol. 14, Issue 1, pp. 130, (2014) (PubMed).

Souza, Silva, Calloway, Kucuk, Rossi, McLemore: "Genistein protects hematopoietic stem cells against G-CSF-induced DNA damage." in: Cancer prevention research (Philadelphia, Pa.), Vol. 7, Issue 5, pp. 534-44, (2014) (PubMed).

Li, Yu, Liu, Zhu, He, Peng, Hua: "miR-34c works downstream of p53 leading to dairy goat male germline stem-cell (mGSCs) apoptosis." in: Cell proliferation, Vol. 46, Issue 2, pp. 223-31, (2013) (PubMed).

Teng, Chang, Wang, Lu, Wu, Yang, Chiu, Yang, Hsu, Ho: "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." in: Biomaterials, Vol. 34, Issue 30, pp. 7462-70, (2013) (PubMed).

Wang, Wu, Gao, Chen, Gu, Ding, Guo: "Elevated toll-like receptor 3 inhibits pancreatic ?-cell proliferation through G1 phase cell cycle arrest." in: Molecular and cellular endocrinology, Vol. 377, Issue 1-2, pp. 112-22, (2013) (PubMed).

Zhang, Tan, Wang, Chen, Quan, Xian, Zhang, He, Zhang: "Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway." in: Experimental cell research, Vol. 320, Issue 1, pp. 119-27, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1 (CCND1 Products)

Synonyms: BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody, AI327039 antibody, CycD1 antibody, Cyl-1 antibody, bcl-1 antibody, cD1 antibody, cb161 antibody, cycd1 antibody, etID37810.7 antibody, fb52e01 antibody, fc45c08 antibody, fc83a12 antibody, id:ibd1198 antibody, wu:fb52e01 antibody, wu:fc45c08 antibody, wu:fc83a12 antibody, ccnd1b antibody, prad1 antibody, ccnd1 antibody, ccnd1a antibody, bcl1 antibody, d11s287e antibody, u21b31 antibody, CCND1 antibody, cyclin D1 antibody, cyclin D1 S homeolog antibody, cyclin D1 L homeolog antibody, CCND1 antibody, Ccnd1 antibody, ccnd1 antibody, ccnd1.S antibody, ccnd1.L antibody

Background:

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1

Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

Gene ID: 12443

UniProt: P25322

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling